Selection and evaluation of Bradyrhizobium inoculum for peanut, Arachis hypogea production in the Lao People’s Democratic Republic

نویسندگان

چکیده

The interaction between leguminous plants and Bradyrhizobium is limited, known as host specificity. Therefore, the selection of an appropriate Bradyrhizobia for use biofertilizer inoculum legumes necessary. Arachis hypogea L. most popular legume produced in Lao People's Democratic Republic (PDR). this research aimed to obtain that provides high efficiency A. production PDR. 14 isolates were obtained from root nodules trapped with PDR soil samples. Three top PMVTL-01, SMVTL-02, BLXBL-03 showing peanut growth promotion. Strains PMVTL-01 SMVTL-02 closely related geno sp. SA-3 Rp7b B. zhanjiangense, respectively, whilst strain was CCBAU51745 manausense BR3351. competitiveness these strains SUTN9-2::GFP analyzed, only performed a number occupied higher than SUTN9-2::GFP. In addition, selected sample pot level employed by tagging DsRed gene. results demonstrated DsRed-expressing tagged showed nodule occupancy indigenous strains. Moreover, acetylene reduction assay (ARA), number, dry weight, total plant weight experiment inoculated SMVTL-02::DsRed presented having potential promote compared non-inoculation. Thus, could be considered

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ژورنال

عنوان ژورنال: Journal of Applied and Natural Science

سال: 2023

ISSN: ['0974-9411', '2231-5209']

DOI: https://doi.org/10.31018/jans.v15i1.4270